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Each PCR product was size-selected on a 2% agarose gel, purified and eluted into 50 l of ddH 2 O using the PCR/Gel Extraction Kit (Nippon Genetics, no

Excess ROS production can increase NF-kB expression in immune cells, leading to excess secretion of pro-inflammatory cytokines, including TNF-a, IL-1, IL-6, IL-8, IFN-g, iNOS, COX-2 [50]