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The cells were seeded in a 6-well plate (Corning) and transfected with the constructs of rat wild-type Tshr ( Tshr WT -GFP ) and truncated Tshr ( Tshr Df -GFP ) in pAcGFP1-N1 (Clontech, 632469) backbone (The cells transfected with GFP expression vector pAAV-GFP was used as negative control)

The GATA4 binding proteins, including p300, RACK1, CDK9, TBX5, Nkx2.5, MEF2, SRF, NFATc4, and HAND2, bind with the C-terminal zinc finger domain of GATA4 (residues 256-326), which includes the multimerization region [13, 14, 17, 37, 44, 45, 66], suggesting that numerous cofactors are concentrated near this region

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4.3.1.2 Common detectors for GC There are many selective detectors such as ECD, nitrogenphosphorous detector (NPD), flame photometric detector (FPD), and flame ionization detector (FID)