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The ashwagandha group was given 300 mg of ashwagandha root extract capsules, twice a day

Rarely, allergic reactions like itching or rash can occur, and excessive or prolonged use may strain the kidneys or liver, especially in those with pre-existing conditions

Proteins required identification with at least two distinct peptides and quantification in at least two technical replicates across four biological replicates, with average intensities calculated for each group

For DBS and VAMS just a simple extraction step with organic solvent mixtures is needed prior to HPLC-MS/MS analysis but, depending on the blood volume sampled, lower analytical performance has to be taken into account (Osteresch et al

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