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J Neuroimmunol 138:99105

N.ZhangJ.RenQ

[116] found that neither folic acid or zinc, taken individually or combined, significantly improved sperm concentration, motility, or morphology

10.1038/s41598-023-49054-9 53 KumarP.OsahonO

The sample was dialyzed against TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) containing decreasing concentrations of NaCl at 1.2, 1, 0.8, and 0.6 M, each for 2 h at 4 C, followed by dialysis against TE overnight at 4 C