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Then, the fluorescent probe DCFH-DA was used to detect the ROS content in zebrafish larvae, and fluorescence intensity was statistically analyzed using ImageJ

1998-2026 Mayo Foundation for Medical Education and Research (MFMER)

Here we demonstrate that the latter approach is preferable, because, owing to the relatively small number of sorted samples used in building panels, standard machine learning does not optimize effect size and cell-type specificity, causing the model to overfit and underperform when tested in independent data