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While necessary, this phase transforms toxins into "reactive intermediate metabolites" that are frequently more toxic and volatile than the original substance, generating massive amounts of free radicals

Fresh DMEM medium was then added, and the cells were exposed to various reagents

EC4 cells were grown in high glucose DMEM supplemented with 10% fetal bovine serum and 1 each of glutamine, nonessential amino acids, and sodium pyruvate