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Subsequently, the leaf apparatus of each plant was separated from the root apparatus, weighed using a precision balance, and finally kept at 20 C for the subsequent biochemical analyses, whereas the root apparatus was first washed thoroughly under running tap H 2 O and then gently rinsed with distilled H 2 O, to remove solid particles, dried with soft paper, and weighed using precision balance

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Blood Adv 1(18):14141422 Charache S, Terrin ML, Moore RD, Dover GJ, Barton FB, Eckert SV, McMahon RP, Bonds DR (1995) Effect of hydroxyurea on the frequency of painful crises in sickle cell anemia