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The suspension was sonicated for 15 min, incubated for 10 min at room temperature, centrifuged (8,000 x g, 30 min, 10 C) and finally freeze-dried overnight

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4.5 Metabolic crosstalk between hepatocellular carcinoma cells and myeloid-derived suppressor cells MDSCs are rapidly expanding in cancer, consisting of two main subgroups: monocytic MDSCs (M-MDSC) and granulocytic MDSCs (G-MDSC), which share the ability to maintain an immature state and suppress adaptive immunity

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