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Description

The BGZ40 cassette contains a human beta-globin minimal promoter, Escherichia coli beta-galactosidase gene ( LacZ ) as a reporter, and a polyadenylation signal (pA) from simian virus 40 (SV40), virtually allowing rigid reporter activation after the transgenesis only when gene regulatory elements (i.e., enhancers) interactive with the human beta-globin minimal promoter were included in the modified BAC clone