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Briefly, cell pellets were resuspended in 30 ml phosphate buffer (100 mM potassium phosphate (PB0445, Biobasic), pH 7.2, 10% glycerol (CA97063-892, VWR)) supplemented with lysozyme (1 mg/ ml) (12650-88-3, Biobasic) and disrupted by sonication

These formulations are often used to help promote smoother-looking skin, improve visible tone, and support a more refreshed, radiant look over time

PEA has shown relevance in inflammation and immune response regulation, and Tributyrin serves as a butyrate source studied for its effects on intestinal epithelial energy metabolism and mucosal integrity

In clinical settings, APS injection is used the most

To view a copy of this license, visit About this article Cite this article Verheijen, M., Lienhard, M., Schrooders, Y