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The pellet was resuspended in L-15 media and filtered through a 2.74 m syringe filter to remove residual cellular debris

Indeed, supplementation with mitoCoQH2 (mitochondria-targeted analogue of CoQH2) suppresses mitochondrial lipid peroxidation and cell death after combined LDHB silencing and RSL3 treatment, consistent with the presence of LDHB in the cell fraction containing the mitochondrial inner membrane

Calibration curves were prepared by seeding known amounts of cells onto 96-well plates

These changes often initiate upwardly spiraling levels of oxidative damage and dysregulation of redox-based cellular communication systems

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