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Combined immunotherapy with granulocyte-macrophage colony-stimulating factor-transduced allogeneic prostate cancer cells and ipilimumab in patients with metastatic castration-resistant prostate cancer: a phase 1 dose-escalation trial

10.2174/1570159x13666160502153022 20 VugmeysterY.XuX.TheilF

The gene ldcC was amplified from the vector pVWEx1- ldcC (Prez-Garca et al., 2018), whereas the different csiD genes were amplified from genomic DNA of the respective organisms and assembled together into BamHI-digested pVWEx1 by Gibson Assembly, using the respective primers