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8 Where results are unclear, serum MMA and homocysteine levels (both of which accumulate with B12 deficiency as discussed above) can also be used to confirm a diagnosis, although homocysteine is not specific to B12 deficiency, as it may also be caused by deficiencies in other B vitamins

A esto se aade el Real Decreto 175/2001, que regula las normas de correcta elaboracin de frmulas magistrales y preparados oficinales en farmacias

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The homogenized tissue was then subjected to centrifugation at 12,000 g for 30 min at 4 C to collect the supernatants