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Limitations : Risk of hypothyroidism requiring lifelong hormone replacement, delayed therapeutic effects, and potential worsening of ophthalmopathy

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Luzindole (LUZ) is a widely used non-selective antagonist of the melatonin receptors MT1 and MT2, enabling us to test whether the effects of exogenous melatonin were receptor-mediated ( Figure 2 Using the same multi-parameter approach applied to the AA model, we evaluated disease outcomes in IL-33 challenged mice in the presence or absence of melatonin and Luzindole

doi: 10.1208/s12248-015-9811-z

Cells were detached 24 h later, fixed, and permeabilized with BD Cytofix/Cytoperm fixation and permeabilization solution according to the manufacturers instructions