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For loss-of-function assays, HASMC WT or T1s were seeded on coverslips at 80% confluence, allowed to adhere, and serum-starved overnight

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However, the blue color should at least partially return when you elute your protein off of the column

5 , e257 (2007)

This multi-symptom, multi-system involvement, and the fact that mast cell activation syndrome is not well-recognized create an extreme challenge for physicians to diagnose