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Y.LuY.SunX

At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS

Kinds of inhibitors showed growth suppressive effect in HCC, including PORCN inhibitors (LGK-974, ETC-159), 331 fungal derivatives (PKF115-854 and CGP0449090), 332,333 TNKS inhibitor XAV939