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To validate the specificity of this interaction, we also generated a murine NKG2D-Ig fusion protein and observed similar C

enhancing protein folding via the inositol-requiring enzyme 1 (IRE1) and X-box binding protein 1 (XBP1) signaling cascade (Ning et al., 2011), and inducing apoptosis and ER-associated degradation by activating transcription factor 6 (ATF6) associated pathway (Lebeaupin et al., 2018)

Implanting surgical tissue directly into mice to generate patient-derived xenografts (PDX) and derive cell lines from PDX tissue is a valid alternative approach to culturing cell lines from surgical tissue directly

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RNA extraction and Real-Time PCR analysis of cytokine expression After separation of DNA and RNA, the latter was further purified using the RNeasy Mini kit (Qiagen) following the suppliers instructions