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The complexes were solubilized from membrane using detergent (1% (w/v) LMNG and 0.06% (w/v) CHS) for 1 h at 4 C

Long-term use of proton-pump inhibitors has been found to decrease blood vitamin B 12 concentrations

Growth hormone exerts both direct and indirect effects on bone metabolism through actions on osteoblasts (bone-forming cells) and osteoclasts (bone-resorbing cells), while IGF-1 serves as a primary mediator of growth hormone's anabolic effects on skeletal tissue

The evidence base is overwhelmingly preclinical, and there are no completed, published Phase 2 or 3 human efficacy trials only early-stage safety work [9]

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