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The following reagents were used: QVD-OPH (BD biosciences), ZVAD-FMK (SelleckChem), Rotenone (Sigma-Aldrich, St

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Previously, we showed this for the left ovarian vein (i.e., inferior caval vein syndrome (Vukojevic et al., 2018)), the inferior mesenteric vein in the portocaval shunt (Strbe et al., 2021)

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Copper Chelation and Delivery At the most fundamental level, GHK functions as a copper chelator and delivery system - the tripeptide binds Cu with exceptionally high affinity (binding constant approximately 10 M, among the highest known for biological copper chelators), forming a square planar coordination complex where the copper ion is coordinated by the nitrogen atoms of the terminal amino group, the two peptide bonds, and the imidazole nitrogen of histidine