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Its what robust science demands

This is largely due to the prevalence of genetic variations in the MTHFR (methylenetetrahydrofolate reductase) gene

Cell grouping and treatment Cigarette smoke extract (CSE) was prepared by bubbling the smoke from two cigarettes into 20 mL of serum-free DMEM, which was then filtered with a 0.2 m filter to sterilize the mixture

J Neurochem 73:14221430 Had-Aissouni L (2011) Toward a new role for plasma membrane sodium-dependent glutamate transporters of astrocytes: maintenance of antioxidant defenses beyond extracellular glutamate clearance