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Lead toxicity induces oxidative stress through generation of ROS (superoxide radicals, hydroxyl radicals)

Next, in all cases, the liposome/protein mixture (final volume 150 L) was adjusted to 28% (w/w) sucrose by mixing 100 L of a 60% (w/w) sucrose solution in HK buffer and overlaid with 200 L of HK buffer containing 24% (w/w) sucrose and 50 L of sucrose-free HK buffer