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This conclusion was supported by comparative genomic, transcriptomic and phenotypic profiles of meningococcal isolates from disease patients and their household contacts, which led to identification of potentially important metabolic differences between carriage and disease isolates including the sulfate assimilation pathway, in addition to the observation that several carriage isolates had lost their type IV pili and that this loss correlated with reduced induction of tumor necrosis factor alpha (TNF-) expression when cultured with epithelial cells (Ren et al., 2017)