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Even small impurities can introduce variability into test results, compromise reproducibility, or interfere with biological activity

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30 it inhibits the attachment of neutrophils to endothelial cells and the expression of adhesion molecules

Then, after rinsing in PBS, the primary antibodies were revealed by applying the appropriate secondary FITC-conjugated antibodies (AlexaFluor 488, 1:500) for 1 h at room temperature