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Statistical analysis Statistical significance was tested by One-way ANOVA with the Tukey test or the unpaired or paired two-tailed Students t-test using JuppyterLite (version 0.6.3), Python (version 3.12.7), and Graph Pad Prism software (version 7.03)

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10.1016/j.jare.2020.07.007 221 WangZ.-K.ChenR.-R.LiJ.-H.ChenJ.-Y.LiW.NiuX.-L.et al (2020b)

We identified a large number of studies, and our results suggest that there is little heterogeneity among the studies

In prepubertal female rats, a cerebroventricular infusion of IGF1 stimulated the secretion of GnRH and might advance the onset of puberty [15, 18]