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Furthermore, among the possible molecular targets of LAs action identified through in silico studies, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and 5-lipoxygenase were highlighted, among other proteins ( Based on this, the objectives of this study were to confirm these potential interaction targets of LA, highlighting its mechanism of action in vitro , and to evaluate LAs role in the oxidative stress imbalance for the prevention of tracheal hyperresponsiveness in asthmatic Wistar rats

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RBC lysis (BioLegend) was performed and CRTH2 + cells were then isolated using the CRTH2 MicroBead Kit (Myltenyi Biotec) according to the manufacturers instructions