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Then, after blocking for 90 min (see Table 2 for details), the membranes were incubated overnight at 4C with the primary antibody, followed by incubation with the secondary antibody

One-way analysis of variance (ANOVA) and unpaired Students t-test were used to compare the study groups

Furthermore on the importance of B12 for your body, your nerves have a protective layer around it, known as a sheath, and B12 helps maintain this sheath and promotes the normal growth of nerves