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In corroboration, we also find a reduction in the total glutathione pool (reduced [GSH] + oxidized [GSSG]), driven by a depletion of GSH upon enolase inhibitor treatment ( Figures 1A , 2C, D )

Confocal imaging of glutathione redox potential in living plant cells

Additionally, we developed a redox-sensitive GFP2-based assay, which allowed the real-time assessment of glutaredoxin structure-function relationships inside living cells

SLC7A11 overexpression SLC7A11 was ectopically expressed in FLO-1, JH-EsoAd1 and H1299 cells using the GE Dharmacon Precision LentiORF pLOC lentiviral vector