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Description

Cells were resuspended in 1 Annexin V Binding Solution to achieve a final concentration of 1 10 6 cells/mL

The NP is tightly enclosed by the circular AF that consists of water, NP cells, notochord cells (NCs), and extracellular matrix (ECM)

As demonstrated in the study by Cristiano et al

(4) mGPDH in the regulation of ferroptosis G3P metabolism supports ferroptosis defense in disease contexts In the first study identifying mGPDH as a ferroptosis regulator, metabolomic analyses revealed that treatment of cancer cells with GPX4 inhibitors led to a marked depletion of intracellular glycerol3phosphate (G3P) 99