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The results of Western blotting confirmed that rapamycin (0.5 nM) significantly reduced phosphorylated S6 expression compared to untreated cells, and the combination of ASP (0.01 mg/mL) and rapamycin showed a more potent inhibitory effect on the expression of phosphorylation of S6 in both cells (Figure 6C)

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This was achieved using the Matlab scripts given in the supplementary software of the Tsigkinopoulou et al protocol

Glutamine deprivation confers immunotherapy resistance by inhibiting ifn- Signaling in cancer cells