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The loss of the chondrocytic phenotype and proliferative potential during in vitro expansion, termed as dedifferentiation, has been well documented

Hint : if performing an analysis of both dried blood spots and whole blood samples in the same microplate, you can start the elution of the dried blood spots and add the elution buffer in the whole blood samples 8 minutes before the elution stage is over

Injection/shot Methotrexate (brand name: Abitrexate, Folex, Mexate) Note: this drug can cause miscarriage and must not be taken during pregnancy because it can cause deformities in the fetus

All subjects provided written informed consent

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