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Reproductive data recorded included sexual activity, fertility desire, previous diagnosis of infertility, obstetric history and the serum level of anti-Mllerian hormone (AMH) prior to the index operation

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Following the reaction, the solution underwent centrifugation at a speed of 12,000 rpm for a duration of 5 min and was subsequently rinsed with methanol in order to acquire the CDCZA nanoparticles composed of Cur/CDDP@Cu/ZIF8@Au

Cells were passaged at approximately 80% confluency using 0.5 mM EDTA (Gibco, AM9260G)

PMID 18493020