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step two, according to the amino acid sequence Fmoc-Thr (tBu) -OH, Fmoc-Asn (Trt) -OH, Fmoc-Ser (tBu) -OH, Fmoc-Gly-OH, Fmoc-Val-OH, Fmoc-Asn (Trt) -OH, Fmoc-Thr (tBu) -OH, Fmoc-Pro-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Pro-OH, Fmoc-Gly-OH, Fmoc-Phe-OH, Fmoc-Asn (Trt) -OH, Fmoc-Ser-Ser (Pro-me) -OH, Fmoc-His (Trt) -OH, Fmoc-Arg (Pbf) -OH, Fmoc-Leu-OH, Fmoc-Phe-OH, Fmoc-Pro-OH, Fmoc-Ser-OH, Fmoc-Ser (Pro-Pro-OH, Fmoc-Pro-OH, Fmoc-Pro-OH, Fmoc-Pro-OH, Fmoc-Pro-OH, Fmoc-Pro-OH, Fmoc-Pro-OH, Fmoc-OH, and Fmoc-Pro-OH, and Fmoc-Pro-OH, and Fmoc-Pro, Fmoc-Glu (OtBu) -OH, Fmoc-Ala-OH, Fmoc-Leu-OH, Fmoc-Arg (Pbf) -OH, Fmoc-Gln (Trt) -OH, Fmoc-Ala-Thr (pro-me-me) -OH, Fmoc-Cys (Trt) -OH, Fmoc-Ala-Thr (pro-me-me) -OH, Fmoc-Thr (tBu) -OH, Fmoc-Asn (Trt) -OH, Fmoc-Cys (Trt) -OH, Fmoc-Lys (Boc) -OH, Fmoc-Glu-OtBu, Eicosaedoic, are coupled as follows: adding DCM into Rink AmideAM resin for swelling, draining the solvent, washing, adding a deprotection reagent to remove Fmoc, and washing to obtain a positive indene detection result

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