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However, after consideration of public comments, discussed in an earlier section of this final rule with comment period, we are finalizing, effective January 1, 2026, our proposal to supplant the existing affirmation requirement with the proposed attestation statement at new ( 180.50(3)(iii), with a modification to add to the following phrase to the beginning of the attestation: To the best of its knowledge and belief,

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Untreated and BSO-treated HeLa cells, infected with PV1-wt (moi 10) were labeled with 35 S-Translabel, as described above, and the cell lysates were analyzed on a 1530% sucrose gradient for the separation of the 75S and 150S intermediates (Materials and Methods)

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Data availability All experimental data are available upon request from the (co-)corresponding authors