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Western blotting For whole-cell protein extraction, cell pellets were lysed in cold RIPA buffer (50 mM TrisHCL pH 7.4, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1 mM Na 3 VO 4 , 0.1% Triton X-100), supplemented before use with cOmplete Protease Inhibitor Cocktail (Roche) and 5 mM sodium butyrate

Recently, it was shown that cysteine uptake via excitatory amino acid carrier 1 suppresses ischemia-induced neuronal death through promotion of hippocampal GSH synthesis in ischemic animal models (162)

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SuperPred is a web-based tool designed to predict the Anatomical Therapeutic Chemical (ATC) classification and potential targets of compounds, whereas STP uses multi-logistic regression models, leveraging 2D and 3D fingerprint similarity to forecast the likely targets of compounds