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Experimental models for predicting drug absorption and metabolism

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These results were largely expected, as previous literature reported chelating interactions between ciprofloxacin and Zn that decrease cellular permeability to fluoroquinolones ( Immediate zinc pre-exposure facilitates accelerated resistance We next performed whole genome sequencing on single-clonal populations of 5-day Zn pre-exposed cells (prior to ciprofloxacin exposure) from three samples, to discern any potential genetic changes that may afford cells the ability to adapt and gain resistance to ciprofloxacin more quickly after Zn pre-exposure (Table 1