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All cells were maintained at 37 C in a humidified atmosphere containing 5% CO

The resulting trimmed and de-duplicated reads were then aligned to the Rattus norvegicus Rnor6.0 reference genome, available on ENSEMBL, using STAR aligner v.2.5.2b4 25 , producing BAM files

doi: Poon HF, Castegna A, Farr SA, Thongboonkerd V, Lynn BC, Banks WA, Morley JE, Klein JB, Butterfield DA (2004) Quantitative proteomics analysis of specific protein expression and oxidative modification in aged senescence-accelerated-prone 8 mice brain

Erastin and RSL3 treatment do not trigger morphological changes or biochemical processes consistent with apoptosis, such as chromatin margination or cleavage of poly ADP-ribose polymerase (PARP) [12, 13, 17]

doi: 10.3390/cancers15020548 247 GuptaPBOnderTTJiangGTaoKKuperwasserCWeinbergRAet al