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There is already ample evidence that ferroptosis does occur during neurodegenerative diseases, and the treatment of neurodegenerative diseases through targeting ferroptosis regulators has proven to be reliable

Annu Rev Biochem 63:915948 Moller P, Wallin H (1998) Adduct formation, mutagenesis and nucleotide excision repair of DNA damage produced by reactive oxygen species and lipid peroxidation product

Quantification of mRNA expression levels of specific targets was evaluated by real-time PCR (RT-PCR) using QuantiFast SYBR Green PCR kit (Qiagen #204054)