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Similarly, polysaccharides prevent the release of inflammatory cytokines in response to external stimuli like lipopolysaccharide (LPS), including interleukin -1 beta (IL-1), intereukin-6 (IL-6), and tumor necrosis factor (TNF-) [25]

The candidate genes underwent Mendelian randomization (MR) analysis and least absolute shrinkage and selection operator analysis to identify key genes

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Overnight incubation of samples at 4 C with anti-TH antibody (Cat:# ab112/1:200/Abcam/MA/USA) was performed, and then, they were washed with phosphate buffer saline (PBS) and incubated for 20 min with HRP Envision antibody kit (DAKO/CA/USA)

Systemic dendrimer delivery of triptolide to tumor-associated macrophages improves anti-tumor efficacy and reduces systemic toxicity in glioblastoma