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FLIM can record fluorescence lifetimes using the time domain technique, time-correlated single-photon counting (TCSPC) (Figure 2 ( Figure 2 Fluorescent substances are excited with a short pulse laser, and the time interval it takes for molecules to excite and emit a photon is captured by a detector, allowing the calculation of the fluorescence lifetime

[DOI] [PMC free article] [PubMed] [Google Scholar] 97.Turk B

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and residues Asp461 and Asp433, the latter being involved in a hydrogen bonding network with the side chains of Lys508 and His547, likely critical for GT2 domain folding stability