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Three modules are GPI-anchored protein signal sequences from DAF, BY55, and CEAM7, and the other three are transmembrane domains (TMDs) from ITAV, ITA5, and ITB3 as used previously ( FIGURE 1 GPI anchoring modules transport Glutathione S-transferase to the cell membrane efficiently We transfected Lenti-X 293T cells with plasmids expressing each GST tag to compare their ability in membrane translocation

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2 Phytochemicals for brain health: promise and challenges 2.1 Major neuroprotective phytochemicals 2.1.1 Curcumin Curcumin, a hydrophobic polyphenol isolated from the rhizomes of Curcuma longa , has attracted considerable interest as a candidate therapeutic agent for NDD including PD, AD, HD, MS, ALS, and prion-associated disorders