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Fractions containing SF3B1 were pooled together, washed with PBS and concentrated in an Amicon Ultra-4 Centrifugal Filter Unit (Sigma-Aldrich, UFC801024)

Sonodynamic therapy (SDT) utilizes ultrasound as the excitation source, offering much greater tissue penetration depth than visible or even near-infrared light, enabling non-invasive treatment of deep-seated tissues (Jiang Z