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For NMR analysis, polar fractions were resuspended in 630 l of PBS (pH 7.4), adding 70 l of 2 H 2 O solution (containing 1 mM sodium 3-trimethylsilyl [2,2,3,3-2H4]propionate (TSP) as a chemical shift reference for 1 H spectra) to provide a field frequency lock, reaching 700 l of total volume

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The actual milligram dose depends entirely on your reconstitution concentration

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