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Zinc release from protein kinase C as the common event during activation by lipid second messenger or reactive oxygen

However, biological effects can extend far beyond the detection window

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The investigators responsible for drug administration, behavioral testing, and outcome assessment were blinded to the group allocation throughout the experiment.To minimize batch effects, all surgical procedures were performed in a randomized order each day

Probucol, a lipid-lowering agent, also exhibits antioxidant properties that reduce intracellular oxidative stress and maintain normal cellular redox state by upregulating Nrf2 and GPX4 protein expression, thereby enhancing cardiomyocyte antioxidant capacity