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Hydrogen peroxide, a ROS of major biological significance, can form as a result of the reaction of superoxide and also can be generated by specific enzymes ( synthesis and its subsequent dismutation to H 2 O 2 in the apoplast, is a common response to pathogens, elicitors, wounding, heat, ultra-violet light, and ozone ( 2 O 2 has a key signaling role in plants ( 2 O 2 can induce gene expression and modulates signaling proteins, such as protein phosphatases (PP), protein kinases (PK), transcription factors and calcium channels that are located in the plasma membrane or elsewhere ( ROS AND NO SIGNALING IN THE HYPERSENSITIVE RESPONSE A well-documented form of plant programed cell death (PCD) is the HR, characterized by the rapid cell death surrounding infection sites

doi: 10.1016/j.tifs.2024.104339 43

The schematic diagram of co-culture system is shown in Figure S1D

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To resolve this issue a 1,000 s dataset was fit to five components and the spectra shown reflect these five states plus one additional final state deconvoluted from the 8,000 s dataset