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As we gain experience with these policies, we may revisit potential interactions between the policies in future notice and comment rulemaking

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Briefly, 25 L reaction volume, cDNA (5 L) was mixed with SYBR-Green mixture (12.5 l), primer (2 l), and RNase free water (5.5 l) (Table S2)

Prior to doseresponse experiments, tissue viability and experimental integrity were confirmed by assessing smooth muscle contractility to KCl and endothelial function through acetylcholine-induced relaxation (Figure S4)