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MTHFR mutations that have the greatest influence on health are: Homozygous A1298C (C1298C) and homozygous C677T (T677T) Compound heterozgous A1298C + C677T Compound heterozgous is considered the most severe or at risk, but it is thought that homozygous mutations can inhibit MTHFR enyzme function by up to a whopping 70% (11)

Thereafter, the animals were intubated with anesthesia maintained using 0.53.0% isoflurane gas