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Likewise, Lu et al

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cDNA equal to 20 ng of total RNA was amplified by qPCR in duplicate in a ViiA7 qPCR system (Thermo Fisher Scientific), using the Fast Advance Master Mix (Thermo Fisher Scientific), assembled with primer and probe sets for VEGF-A and GFAP genes (IDs: Mm0043736_m1 and Mm01253033_m1 respectively) and one housekeeping gene, 18s rRNA (ID: 4333760 F) (Thermo Fisher Scientific)

In one study, pressure inside a spinal disc dropped to below 100 mm Hg when VAX-D was applied, showing that the treatment can effectively decompress the discs (Ramos et al., 1994)

SS rats exhibit significant changes in plasma amino acid levels and skeletal muscle amino acid metabolism compared with SS.13 BN rats or in response to a high-salt diet, especially glycine, serine, and threonine metabolism and alanine, aspartate, and glutamate metabolism 93,109