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The PVDF membranes were treated with a chemiluminescent substrate (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a ChemiDoc Imaging system and Image Lab software (Bio-Rad, Hercules, CA, USA)

To facilitate screening, a co-conversion strategy with dominant phenotypic roller marker was used 62

Overall, our observations imply the relevance of JMF4073 sensitivity to high replication stress associated with low dTTP/GSH levels in WT-Bcr-Abl-32D cells

This is a technique step, not just a cleanliness step

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