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The functional annotation analysis identified, as highly enriched cluster related to GTPase activity, cytoskeletal binding, protease inhibitor, catalytic activity, transporter activity, protein-tyrosine kinase activity, and SOD (Fig

While p53 modulators, including FOXO4-DRI, MDM2 inhibitors, and USP7 inhibitors have shown senolytic effects by selectively eliminating senescent cells or promoting their apoptosis in the laboratory, translating these findings to the clinic have so far been unsuccessful

Expression and purification of recombinant methyltransferase proteins NSD1(SET) (amino acids 18532093, NCBI sequence: NC_000005.10), NSD2(SET) (amino acids 9581365, NCBI sequence: NC_000004.12), NSD3(SET) (amino acids 10211320, NCBI sequence: NC_000008.11), ASH1L(SET) (amino acids 19802564, NCBI sequence: NC_000001.11), SETD2(SET) (amino acids 13232564, NCBI sequence: NC_000003.12) and SUV39H1(SET) (amino acids 82412, NCBI sequence: NC_000023.11) were cloned into pGEX-6P-1

At acidic pH (below 5), deamidation occurs through direct hydrolysis and is generally slower