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designed a series of nucleoside-derived inhibitors, among which compound 6 demonstrated exceptional inhibitory activity in the double-digit nanomolar range against the N 7-MTase NSP14

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For whole-mount immunofluorescence and confocal imaging, organoids were fixed in 4% PFA for 60 minutes, pelleted (300x g for 5 min, low deceleration) and washed in PBS for 20 min, which was repeated three times